TP-FLIM双光子荧光寿命成像光学造影剂

双光子荧光寿命成像（Two-photon fluorescence lifetime imaging, TP-FLIM）技术集合了双光子荧光成像和荧光寿命成像的优势，能够对生物组织进行高分辨三维成像的同时提供生物组织的生化特性信息，在**诊疗方面具有巨大的临床应用前景。为了获得较佳的双光子荧光寿命成像效果，光学造影剂除了需要具有较强的双光子吸收，用于降低激光的能量，避免高能量激光对组织的损伤；还需要具有稳定且较长的荧光寿命，用于去除生物自荧光对双光子荧光寿命成像的干扰。然而，目前还鲜有材料能够同时满足上述要求。因此，迫切需要设计合成一种合适光学造影剂，用于推动双光子荧光寿命成像技术在生物诊疗领域的发展。

一种基于热激活延迟荧光（Thermally activated delayed fluorescence, TADF）的有机光学造影剂，可用于双光子活体荧光寿命成像。在该工作中，研究者充分利用热激活延迟荧光材料通常具有较长延迟荧光（Delayed fluorescence）寿命这一优点，将具有聚集诱导荧光增强的有机热激活延迟荧光材料包覆于两亲性共聚物内，成功制备了水溶性的有机纳米颗粒。光学研究表明两亲性共聚物的包覆较大化了热激活延迟荧光材料的聚集态，大大增加了材料的荧光寿命，获得了可媲美磷光材料的超长发光寿命（4.2 μs）。更为重要的是，不像传统磷光发光强度和寿命容易被氧气猝灭，该材料对氧气表现出明显的惰性，即便在富氧区域仍然表现出稳定的荧光寿命，非常有利于获得高信噪比的荧光寿命成像。同时，该热激活延迟荧光材料由于具有Donor-Acceptor（D-A）结构，表现出良好的双光子吸收截面，轻易实现了活体双光子荧光寿命成像。

该研究为双光子荧光寿命成像技术提供了一种良好的光学造影剂，对推动双光子荧光寿命成像技术在临床**诊疗中的发展具有重要意义。

活体荧光造影剂    

Sulfo-CY7(Cyanine7)NHSester    

Sulfo-Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

sulfo-CY7(Cyanine7)maleimide    

sulfo-Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)dicarboxylicacid    

Sulfo-Cyanine7.5dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)carboxylicacid    

Sulfo-Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)azide    

Sulfo-Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)alkyne    

Sulfo-Cyanine7.5alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)tetrazine    

Sulfo-Cyanine7tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)NHSester    

Sulfo-Cyanine7NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)maleimide    

Sulfo-Cyanine7maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

sulfo-CY7(Cyanine7)dicarboxylicacid    

sulfo-Cyanine7dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)carboxylicacid    

Sulfo-Cyanine7carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)bis-NHSester    

Sulfo-Cyanine7bis-NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)azide    

Sulfo-Cyanine7azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)amine    

Sulfo-Cyanine7amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)alkyne    

Sulfo-Cyanine7alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)tetrazine    

Cyanine7.5tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)NHSester    

Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)maleimide    

Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)hydrazide    

Cyanine7.5hydrazide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)carboxylicacid    

Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide    

Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide,10mM/DMSO    

Cyanine7.5azide,10mM/DMSO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)amine    

Cyanine7.5amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)alkyne    

Cyanine7.5alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)tetrazine    

Cyanine7tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)NHSester    

Cyanine7NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)maleimide    

Cyanine7maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)hydrazide    

Cyanine7hydrazide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)dicarboxylicacid    

Cyanine7dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)DBCO    

Cyanine7DBCO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)carboxylicacid    

Cyanine7carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide    

Cyanine7azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide,10mM/DMSO    

Cyanine7azide,10mM/DMSO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)amine    

Cyanine7amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)alkyne    

Cyanine7alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

BDP650/665XNHSester    

BDP650/665XNHSester,Borondipyrromethenedyeforfluorescein(516nm)channel-verybrightandphotostablefluorophore.    

BDP650/665maleimide    

BDP650/665maleimide,Borondipyrromethenedyeforfluorescein(515nm)channel-verybrightandphotostablefluorophore.    

BDP650/665azide    

BDP650/665azide,Borondipyrromethenedyeforfluorescein(514nm)channel-verybrightandphotostablefluorophore.    

BDP650/665alkyne    

BDP650/665alkyne,Borondipyrromethenedyeforfluorescein(513nm)channel-verybrightandphotostablefluorophore.    

BDP630/650XNHSester    

BDP630/650XNHSester,Borondipyrromethenedyeforfluorescein(512nm)channel-verybrightandphotostablefluorophore.    

BDP630/650tetrazine    

BDP630/650tetrazine,Borondipyrromethenedyeforfluorescein(511nm)channel-verybrightandphotostablefluorophore.    

BDP630/650maleimide    

BDP630/650maleimide,Borondipyrromethenedyeforfluorescein(510nm)channel-verybrightandphotostablefluorophore.    

BDP630/650hydrazide    

BDP630/650hydrazide,Borondipyrromethenedyeforfluorescein(509nm)channel-verybrightandphotostablefluorophore.    

BDP630/650carboxylicacid    

BDP630/650carboxylicacid,Borondipyrromethenedyeforfluorescein(508nm)channel-verybrightandphotostablefluorophore.    

BDP630/650azide    

BDP630/650azide,Borondipyrromethenedyeforfluorescein(507nm)channel-verybrightandphotostablefluorophore.    

BDP630/650amine    

BDP630/650amine,Borondipyrromethenedyeforfluorescein(506nm)channel-verybrightandphotostablefluorophore.    

BDP630/650alkyne    

BDP630/650alkyne,Borondipyrromethenedyeforfluorescein(505nm)channel-verybrightandphotostablefluorophore.    

上述产品齐岳生物均可供应，仅用于科研！

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