不同DSPE-PEG构型对纳米粒子性能的影响对比-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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不同DSPE-PEG构型对纳米粒子性能的影响对比

发布时间：2025-06-26 作者：zyl 分享到：

文献：Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

文献链接：https://www.tandfonline.com/doi/full/10.2147/IJN.S152312#d1e205

作者：Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su

摘要：

Preparation and characterization of pH-sensitive DSPE-PEG-Imide-MTX assembling micellar nanoparticles loaded with CUR (MTX-Imine-M-CUR)

Because of inherent amphiphilicity, DSPE-PEG-Imine-MTX and DSPE-MPEG could spontaneously self-assemble into micellar nanoparticles (MTX-Imine-M) composed of an inner hydrophobic DSPE core and an outside hydrophilic PEG shell with a self-targeting prodrug corona when exposed to the aqueous solution. The CUR drug could be encapsulated within the hydrophobic core of MTX-Imine-M (MTX-Imine-M-CUR) through hydrophobic interactions. The MTX-Imine-M-CUR nanoparticles were characterized by dynamic light scattering (DLS) and transmission electron microscope (TEM). In addition, the M-CUR (nanocarriers were self-assembled by DSPE-MPEG) and the MTX-Amide-M-CUR (nanocarriers were self-assembled by DSPE-PEG-Amide-MTX and DSPE-MPEG) were fabricated as comparative formulations (Figure 1B). The TEM observation of all drug formulations (Figure 2A–C) confirmed the monodispersed spherical nanoparticles. With DLS measurement, all nanoparticles showed a mean hydrodynamic particle size of 20–30 nm with negative surface charge (Figure 2A–C), which might facilitate preventing potential aggregations via electrostatic repulsion and decrease the nonspecific interaction between nanoparticles and plasma protein/red blood cells.

由于其固有的两亲性，当暴露于水溶液中时，DSPE-PEG-Imine-MTX和DSPE-MPEG可以自发地自组装成胶束纳米粒子（MTX-Imine-M），该纳米粒子由内部疏水性DSPE核心和外部亲水性PEG外壳组成，具有自靶向前药冠。

CUR药物可以通过疏水相互作用包封在MTX-Imine-M（MTX-Imine-M-CUR）的疏水核心内。通过动态光散射（DLS）和透射电子显微镜（TEM）对MTX-Imine-M-CUR纳米粒子进行了表征。

此外，M-CUR（纳米载体由DSPE-MPEG自组装）和MTX-酰胺-MCUR（纳米粒子由DSPE-PEG-酰胺-MTX和DSPE-MPEG自行组装）被制备为比较制剂。所有药物制剂的TEM观察证实了单分散的球形纳米颗粒。通过DLS测量，所有纳米粒子的平均流体动力学粒径为20-30nm，表面带负电荷，这可能有助于通过静电排斥防止潜在的聚集，并减少纳米粒子与血浆蛋白/红细胞之间的非特异性相互作用。