文献：Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery

作者：King Sun Siu, Yujuan Zhang, Xiufen Zheng, James Koropatnick & Wei-Ping Min

文献链接：https://link.springer.com/protocol/10.1007/978-1-4939-3112-5_13

摘要：

The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.

小干扰RNA (SiRNA) 可以特异性地下调基因表达。修饰碳纳米管 (CNT) 可用于保护 SiRNA 并促进其进入细胞。

尽管如此，仍然缺乏简单有效的 CNT 功能化方法。使用非共价功能化的 CNT 可以实现有效的 SiRNA 递送，其中非共价（相对于共价）功能化更简单、更快捷。

本文介绍了包含脂质聚合物的非共价功能化的单壁碳纳米管 (SWCNT)。生成了与 1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N- [氨基（聚乙二醇）-2000] (DSPE-PEG) 偶联的聚乙烯亚胺 (PEI)，并用该产物分散 CNT 形成 DSPE-PEG-PEI/CNT (DGI/C)，这是一种能够促进 SiRNA 递送至体外细胞以及体内器官和细胞的试剂。

相关推荐产品：

DSPE-PEG-NBD

DSPE-PEG-NPC

DSPE-PEG-NTA-NI

DSPE-PEG-PCL

DSPE-PEG-PDP

DSPE-PEG-PEI

DSPE-PEG-PLA

DSPE-PEG-PLGA

DSPE-PEG-Progestrone

DSPE-PEG-RGD

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