文献：DSPE-PEG聚合物改善大鼠难吸收大分子肺吸收及相关机制

链接：https://www.tandfonline.com/doi/abs/10.1080/03639045.2021.1879837

作者：杨高，亚孙，廖广利，张海龙&龙庆志

摘要：

本研究旨在探讨DSPE-PEG聚合物（DSPE-PEG-OH和DSPE-PEG-SH）通过肺内给药改善难吸收大分子吸收的潜力及其潜在机制。

方法

为探究模型化合物经大鼠肺内给药后的吸收情况，开展了大鼠在体肺吸收实验。通过肺组织形态学观察和支气管肺泡灌洗液（BALF）中生物毒性标志物的测定，评估了DSPE-PEG聚合物对大鼠肺组织局部膜损伤的影响。采用荧光偏振法和蛋白质印迹法分别研究了这些聚合物对肺膜流动性和紧密连接相关蛋白基因表达的影响，探讨了其促进吸收的潜在机制。

Abstract

Objective

This study aims to investigate the potential of DSPE-PEG polymers (DSPE-PEG-OH and DSPE-PEG-SH) on improving absorption of poorly absorbable macromolecules via intrapulmonary administration and underlying mechanism.

Methods

In situ pulmonary absorption experiments were performed to investigate the absorption of model compounds after intrapulmonary administration to rats. The local membrane damage induced by these DSPE-PEG polymers were evaluated based on morphological observation of lung tissues and measurement of biological toxic markers in bronchoalveolar lavage fluid (BALF) postintrapulmonary delivery of DSPE-PEG polymers to rats. The underlying enhancement mechanism of these polymers was explored by investigating their effects on the pulmonary membrane fluidity and gene expression of tight junction associated proteins with fluorescence polarization and western blotting, respectively.

西安齐岳生物提供相关产品：

DSPE-COOH，DSPE-Succinic acid

DSPE-MAL，DSPE-Maleimide

DSPE-NHS

DSPE-Hyaluronate

DSPE-Dextran

DSPE-Chitosan

DSPE-RB

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