文献：Following the Fate of Dye-Containing Liposomes In Vitro

作者：by Jennifer Cauzzo 1ORCID,Mona Nystad 2,3ORCID,Ann Mari Holsæter 1,Purusotam Basnet 2,4ORCID andNataša Škalko-Basnet 

文献链接：https://www.mdpi.com/1422-0067/21/14/4847

To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to follow the nanocarrier internalization and targeting; however, the validity of the obtained results strictly depends on how much the nanosystem’s fate can be inferred from the fate of fluorescent dyes. To evaluate the parameters that affect the physicochemical and biological stability of the labeled nanosystems, we studied the versatility of two lipid dyes, TopFluor®-PC and Cy5-DSPE, in conventional liposomes utilizing well-defined in vitro assays. Our results suggest that the dye can affect the major characteristics of the system, such as vesicle size and zeta-potential. However, a nanocarrier can also affect the dye properties. Medium, temperature, time, fluorophore localization and its concentration, as well as their interplay, affect the outcome of tracing experiments. Therefore, an in-depth characterization of the labeled nanosystem should be fundamental to understand the conditions that validate the results within the screening process in optimization of nanocarrier.

为了确定影响纳米系统作为药物载体和潜在纳米药物适用性的因素，我们专注于通过基于荧光的检测，即流式细胞术和成像来追踪它们的命运。

这些方法通常用于跟踪纳米载体的内化和靶向；然而，所得结果的有效性严格取决于纳米系统的命运在多大程度上可以从荧光染料的命运中推断出来。

为了评估影响标记纳米系统的物理化学和生物稳定性的参数，我们利用明确的体外试验研究了两种脂质染料TopFluor®-PC和Cy5-DSPE在传统脂质体中的通用性。

我们的结果表明，染料会影响系统的主要特征，如囊泡大小和ζ电位。然而，纳米载体也会影响染料性能。介质、温度、时间、荧光团定位及其浓度，以及它们的相互作用，都会影响示踪实验的结果。因此，对标记纳米系统的深入表征应该是理解在优化纳米载体的筛选过程中验证结果的条件的基础。

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