mPEG-DSPE/阿霉素胶束的制备及其淋巴结转移治疗效果研究-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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mPEG-DSPE/阿霉素胶束的制备及其淋巴结转移治疗效果研究

发布时间：2025-07-09 作者：kx 分享到：

文献：MPEG-DSPE聚合物胶束用于淋巴结转移的经淋巴化疗

链接：https://www.sciencedirect.com/science/article/abs/pii/S0378517315002902

作者：雪莉，董青，严志强，维岳路，冯玲玲，曹协阿，谢作旭，苏冰霞，刘敏阿

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本研究制备了甲基聚乙二醇-二硬脂酰磷脂酰乙醇胺/阿霉素（MPEG-DSPE/DOX）胶束用于*淋巴结转移。制备的MPEG-DSPE/DOX胶束呈球形，粒径为20 ± 5nm。A375细胞对DOX和MPEG-DSPE/DOX胶束的摄取率分别为51.2%和88.7%。RAW264.7细胞对MPEG-DSPE/罗丹明B胶束的吞噬率比单独使用罗丹明B低17.2倍。皮下注射后，MPEG-DSPE胶束经淋巴管吸收，在腘窝淋巴结中聚集。 MPEG-DSPE/DOX胶束较单独DOX能明显减轻注射部位皮下组织的损伤。建立A375细胞淋巴结转移裸鼠模型，皮下注射后，MPEG-DSPE/DOX胶束组腘窝淋巴结和髂窝淋巴结重量均明显低于生理盐水组和DOX组。MPEG-DSPE/DOX胶束能有效杀伤腘窝淋巴结和髂窝淋巴结中的*细胞。综上所述，MPEG-DSPE胶束是一种很有前景的*淋巴结转移的药物递送系统。

Abstract

Lymph node metastasis is one of the major pathways for tumor formation and it is difficult to deliver chemotherapeutics at therapeutic concentrations to lymph node metastasis. This study prepared methyl poly(ethylene glycol)-distearoylphosphatidylethanolamine/doxorubicin (MPEG-DSPE/DOX) micelle for the treatment of lymph node metastasis. The MPEG-DSPE/DOX micelle prepared were of spherical morphology with a particle size of 20 ± 5 nm. The uptake rates of DOX and MPEG-DSPE/DOX micelle by A375 cells were 51.2% and 88.7%, respectively. The phagocytosis rate of MPEG-DSPE/Rhodamine B micelle by RAW264.7 cells was 17.2-fold lower than for Rhodamine B alone. After subcutaneous injection, MPEG-DSPE micelle underwent lymphatic absorption and accumulated in popliteal lymph nodes. MPEG-DSPE/DOX micelle significantly alleviated damage to the subcutaneous tissue of the injection sites compared with DOX alone. We established a model of nude mice bearing lymph node metastasis of A375 cells. After subcutaneous injection, the weights of both the popliteal and iliac lymph nodes of the MPEG-DSPE/DOX micelle group were significantly lower than in the saline and DOX groups. MPEG-DSPE/DOX micelle effectively killed the tumor cells in popliteal and iliac lymph nodes. In conclusion, MPEG-DSPE micelle is a promising drug delivery system for the treatment of lymph node metastasis.

mPEG-DSPE