DSPE-PEG-COOH封端脂质体表面偶联PPACK肽的策略及其稳定性研究-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

您当前所在位置：首页 > 资讯信息 > 试剂课堂

产品分类

热销试剂

新品上市

DSPE-PEG-COOH封端脂质体表面偶联PPACK肽的策略及其稳定性研究

发布时间：2025-07-09 作者：zyl 分享到：

文献：BioMed Research International

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1155/2014/129458

摘要：

Postmodification of the liposome surface by amine-carboxyl conjugation (Figure 1(b)) was recently reported to overcome the rapid systemic clearance of D-phenylalanyl-L-prolyl-L-arginyl-chloromethyl ketone short peptide (PPACK), an antithrombin agent [30]. Palekar et al. demonstrated that PPACK peptide is attached to the surface of preformed liposomes composed of EPC, DPPE, and DSPE-PEG2000-COOH (94 : 4 : 2 molar ratio) by applying standard amine-carboxyl coupling conditions for conjugating the N-terminus of the peptide to preformed carboxy-terminated DSPE-PEG-containing liposomes. The peptide was conjugated to the unilamellar liposomes and liposomes were purified by dialysis for four hours, which suggests high stability of the nanosystem.

通过胺羧基偶联对脂质体表面进行后修饰，可以克服抗凝血酶剂D-苯丙氨酰-L-脯氨酰基-L-精氨酰氯甲基酮短肽（PPACK）的快速全身清除。Palekar等人通过应用标准胺羧基偶联条件将肽的N端偶联到预先形成的含羧基封端的DSPE-PEG的脂质体上，证明了PPACK肽附着在由EPC、DPPE和DSPE-PEG2000-COOH（94:4:2摩尔比）组成的预先形成的脂质体的表面上。肽与单层脂质体结合，脂质体通过透析4小时纯化，这表明纳米系统具有很高的稳定性。