文献：BMS-1166靶向递送增强乳腺癌免疫*

链接：https://www.tandfonline.com/doi/full/10.2147/IJN.S497089#d1e381

作者：于哲成，周泽亚&赵云琪

节选：

方法

将BMS-1166封装于T7-PEG-PCL胶束中。通过动态光散射测定粒径和zeta电位。通过透射电子显微镜研究颗粒形貌。通过傅里叶变换红外光谱、热重分析和差示扫描量热法对颗粒进行表征。通过高效液相色谱检测药物包封率、载药量和释放曲线。以人乳腺癌MDA-MB-231细胞进行细胞毒性试验。采用流式细胞术和免疫荧光成像研究细胞表面和外泌体中PD-L1的抑制情况。MDA-MB-231与Jurkat共培养研究T细胞活化和凋亡情况。

Methods

BMS-1166 was encapsulated in T7-PEG-PCL micelle. Particle size and zeta potential were determined by dynamic light scattering. Particle morphology was studied by transmission electron microscopy. The particles were characterized by Fourier transform infrared, thermogravimetric analysis, and differential scanning calorimetry. Drug encapsulation efficiency, loading degree, and release profile were examined by high-performance liquid chromatography. Human breast cancer MDA-MB-231 was used to test the cytotoxicity. Flow cytometry and immunofluorescence imaging were used to study the PD-L1 inhibition in cell surface and exosomes. MDA-MB-231 and Jurkat co-culture studied T-cell activation and apoptosis.

西安齐岳生物提供相关产品：

DSPE-PEG-Antinucleosome mAb 2C5（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-PEI-Dextran

mPEG-DOPE

DSPE-PEG-YCAREPPTRTFAYWG（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-PEG-COOH

DSPE-PEG-FSHB(二硬脂酰基磷脂酰乙醇胺-聚乙二醇-卵巢癌靶向肽)

DSPE-PEG-Mannose,DSPE-PEG-Man

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