Bi（mPEG-S-S）-TPE胶束的制备与表征-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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Bi（mPEG-S-S）-TPE胶束的制备与表征

发布时间：2025-07-14 作者：zyl 分享到：

文献：AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery

作者：by Wei Zhao 1,†,Zixue Li 1,†,Na Liang 2,*,Jiyang Liu 1,Pengfei Yan 1 andShaoping Sun

文献链接：https://www.mdpi.com/1422-0067/23/18/10801

The drug-loaded Bi(mPEG-S-S)-TPE micelles were obtained as follows. At first, the Bi(mPEG-S-S)-TPE dispersion in distilled water was prepared. Then, the PTX in the acetone solution was added. After being sonicated for 3 min (on for 3 s and off for 2 s), the mixture was dialyzed (MWCO of 3500 Da) against distilled water for 2 h and further filtrated with a 0.45 μm filter to obtain the drug-loaded micelles. The micelles could be further freeze-dried and stored as a white powder.

XRD analysis was used to analyze the crystalline characteristic of PTX in the micelles (Geigerflex, Rigaku Co., Tokyo, Japan). Data were collected from 5° to 50° with a step-scan mode. The dynamic light-scattering method was employed to determine the particle size and zeta potential of PTX-loaded micelles (Zetasizer Nano-ZS90, Malvern Instruments, Malvern, UK). The drug loading (DL) and encapsulation efficiency (EE) were calculated using Equations (2) and (3), respectively.

Bi（mPEG-S-S）-TPE胶束的制备与表征

载药的Bi（mPEG-S-S）-TPE胶束如下获得。首先，制备了Bi（mPEG-S-S）-TPE在蒸馏水中的分散体。然后，加入丙酮溶液中的PTX。经过3分钟的超声处理（开启3秒，关闭2秒）后，将混合物用蒸馏水透析（MWCO为3500 Da）2小时，然后用0.45μm过滤器进一步过滤，得到载药胶束。胶束可以进一步冷冻干燥，并以白色粉末的形式储存。

XRD分析用于分析胶束中PTX的结晶特性。采用阶跃扫描模式从5°到50°收集数据。采用动态光散射法测定PTX负载胶束的粒径和ζ电位（Zetasizer Nano-ZS90，Malvern Instruments，英国Malvern）。分别使用方程式（2）和（3）计算药物负载量（DL）和包封效率（EE）。