文献：甲胎蛋白*体修饰荧光磁性探针对HepG2细胞及*模型小鼠的 研究

链接：https://www.ingentaconnect.com/contentone/asp/jnn/2020/00000020/00000008/art00088

作者：陈艳帆，卢江环，杨杰，郝凯旋，李敏

节选：

为构建一种双靶向（磁靶向和*体靶向）多功能磁性荧光脂质体探针，该探针具有良好的生物相容性，对早期肝*的诊断具有较高的特异性。称取1，2-二硬脂酰-sn-甘油-3-磷酸-乙醇胺-N- [氨基（聚乙二醇）]（DSPE-PEG2000-NH2）与Cy7 N-羟基琥珀酰亚胺酯（Cy7-NHS）进行反应，生成DSPE-PEG 2000 -Cy7。将蛋黄卵磷脂、胆固醇、DSPE-PEG 2000 -Cy7和Fe 3 O 4依次加入到反应液中，合成荧光四氧化三铁（Fe 3 O 4）脂质体。将磁性脂质体（MLP）与甲胎蛋白（AFP）多克隆*体偶联，并对末端AFP*体修饰的磁性脂质体在人肝*细胞HepG2和肝*模型小鼠中的应用进行了分析研究。荧光四氧化三铁脂质体呈球形，平均流体动力学直径约150nm，带负电荷。该荧光磁性探针对HepG2细胞毒性较小，并提高了AFP*体靶向成像在肝*模型小鼠肝*诊断中的特异性。总之，AFP*体偶联的荧光磁性探针是一种很有前途的多功能工具，具有良好的生物相容性，并具有较高的灵敏度和特异性，可用于临床*症的诊断和*。

To construct a dual-targeting (magnetic targeting and antibody targeting) multi-functional magnetic fluorescent liposome probe with a good biocompatibility and high specificity for early liver cancerdiagnosis. 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[amino(polyethylene glycol)] (DSPE-PEG2000-NH2) and Cyanine7 N-hydroxysuccinimide ester (Cy7-NHS) were measured and reacted for forming DSPE-PEG2000-Cy7. Egg yolk lecithin, cholesterol, DSPE-PEG2000-Cy7 and Fe3O4 were added in orderly into the reaction solution for synthesis of fluorescent ferroferric oxide (Fe3O4) liposome. The Magnetic liposome (MLP) was coupled with Alpha-Fetoprotein (AFP) polyclonal antibody and the ending AFP antibody modified magnetic liposome was analyzed and studied on human hepatoma cell HepG2 and cancer model mouse. The fluorescent ferroferric oxide liposome appeared as a sphericalmorphology and they were about 150 nm in mean hydrodynamic diameter with negative charge. The fluorescent magnetic probe had a little toxic effect on hepG2 cells and increased the specificity liver cancer diagnosis by AFP antibody target imaging in model mouse of liver cancer. In brief, the AFP antibody conjugated fluorescent magnetic probe is a promising multi-functional tool with good biocompatibility and high sensitivity and specificity for clinical cancer diagnosis and therapy.

西安齐岳生物提供相关产品：

DSPE-PEG-Mal

DSPE-RB

DSPE-PEI-Dextran

LyP-1-PEG-DSPE

Angiopep-2 PEG

DSPE-PEG-CSNRDARRC（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-PEG-BSA

以上文章内容来源各类期刊或文献，如有侵权请联系我们删除！