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双功能聚合物 COOH-PEG-SH 在银纳米粒子生物偶联中的应用
发布时间:2025-07-23     作者:zyl   分享到:

文献:Plasmon-enhanced fluorescence provided by silver nanoprisms for sensitive detection of sulfide

作者:G Lin,M Lewandowska

文献链接:https://www.zhangqiaokeyan.com/academic-journal-foreign_sensors-actuators-chemical_thesis/0204120256992.html

摘要

Noble metallic nanostructures, such as silver, play an important role in fluorescent enhancement. In this paper, a serial of silver nanoprisms were fabricated by using seed-mediated growth method, and their surface plasmon resonance (SPR) peak could be controlled from 500 nm to 900 nm through adjusting the volume of silver seed solution. Then three kinds of as-prepared silver nanoprisms were conjugated with Atto550 by using polymer COOH-PEG-SH and streptavidin-biotin bond. It is proved that all these three silver nanoprisms present good ability in enhancing the fluorescent intensity of Atto550, especially the one with SPR peak at 570 nm, which results in almost ten times enhancement of the fluorescent intensity. By using Atto550 functionalized silver nanoprisms (SPR 570 nm), an "off" sensor has been designed, which shows excellent ability in quantitatively detecting sulfide in aqueous solution.

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贵金属纳米结构,如银,在荧光增强中发挥着重要作用。在本文中,通过使用种子介导的生长方法制造了一系列银纳米棱镜,并且通过调节银种子溶液的体积,它们的表面等离子体共振(SPR)峰值可以从500nm到900nm控制。然后通过使用聚合物COOH-PEG-SH和链霉抗生物素蛋白 - 生物素键将三种用制备的银纳米纳压纳米与ATTO550缀合。

事实证明,所有这三种银纳莫纳莫型在增强ATTO550的荧光强度方面存在良好的能力,尤其是在570nm处具有SPR峰的荧光强度,这导致荧光强度的近10倍。通过使用Atto550官能化银纳米素(SPR 570nm),设计了“OFF”传感器,其显示出优异的在水溶液中定量检测硫化物的优异能力。

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