DSPE-PEG2000-MAL在多种纳米杂化物构建中的应用-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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DSPE-PEG2000-MAL在多种纳米杂化物构建中的应用

发布时间：2025-07-03 作者：zyl 分享到：

文献：Dual Functioned Hexapeptide-Coated Lipid-Core Nanomicelles Suppress Toll-Like Receptor-Mediated Inflammatory Responses through Endotoxin Scavenging and Endosomal pH Modulation

作者：Yuting Ji, Liya Sun, Yuan Liu, Yanhui Li, Tongxuan Li, Jiameng Gong, Xiali Liu, Huiqiang Ma, Jingying Wang, Bing Chen, Shan-Yu Fung, Hong Yang

文献链接：

https://advanced.onlinelibrary.wiley.com/doi/full/10.1002/advs.202301230

摘要：

To overcome this problem, we constructed three versions of peptide (Pep12)-modified nano-hybrids by replacing the GNP core with different cores that were made of clinically applicable materials in this study (Figure 1a): M-P12, Lipo-P12, and PLGA-P12. We anticipated that these new cores may also bring additional functionality to the nano-hybrids. M-P12 was made of self-assembled distearoyl-phosphatidylethanolamine-poly(ethylene glycol) (2000)-maleimide (DSPE-PEG2000-MAL) nanomicelles (M-MAL) that were conjugated with Pep12 (CLPFFD) on the surface by Michael addition reaction between the maleimide of the DSPE-PEG2000-MAL and the thiol group of the cysteine (C) residue at the N-terminal of Pep12 (Figure 1b). The nuclear magnetic resonance hydrogen spectroscopy (1H NMR) was applied to confirm the conjugation of Pep12 on the nanomicelle surface. The disappearance of the maleimide peak (at 6.7 ppm) in DSPE-PEG2000-MAL and the appearance of the benzene peak of the peptide (at 7.1–7.2 ppm) in DSPE-PEG2000-Pep12 suggested the complete conjugation of Pep12 to the nanomicelle (Figure S1a, Supporting Information). Lipo-P12 was fabricated by inserting DSPE-PEG2000-Pep12 into the phospholipid bilayer of the DSPE liposomes (Lipo). PLGA-P12 was constructed by conjugating Pep12 to PLGA monomers via amide bond formation (Figure 1b), followed by nano-precipitation to form peptide-modified PLGA nanoparticles. The appearance of the benzene peak in the NMR spectra of PLGA-P12 suggested the successful conjugation of Pep12 to PLGA (Figure S2a, Supporting Information).

DSPE-PEG2000-MAL

我们构建了三种版本的肽（Pep12）修饰的纳米杂化物，通过用本研究中临床适用材料制成的不同核替换GNP核：M-P12、Lipo-P12和PLGA-P12。

我们预计这些新内核也可能为纳米混合动力车带来额外的功能。M-P12由自组装的二硬脂酰磷脂酰乙醇胺聚乙二醇（2000）-马来酰亚胺（DSPE-PEG2000-MAL）纳米胶束（M-MAL）制成，通过DSPE-PEG2000/MAL的马来酰亚胺与Pep12 N端半胱氨酸（C）残基的巯基之间的迈克尔加成反应，在表面与Pep12（CLPFD）偶联。核磁共振氢谱（1H NMR）用于确认Pep12在纳米胶束表面上的共轭。

DSPE-PEG2000-MAL中马来酰亚胺峰（6.7 ppm）的消失和DSPE-PEG2000/Pep12中肽的苯峰（7.1-7.2 ppm）的出现表明Pep12与纳米胶束完全结合。

Lipo-P12是通过将DSPE-PEG2000-Pep12插入DSPE脂质体（Lipo）的磷脂双层中制备的。PLGA-P12是通过酰胺键形成将Pep12偶联到PLGA单体上构建的，然后进行纳米沉淀形成肽修饰的PLGA纳米颗粒。PLGA-P12的NMR光谱中苯峰的出现表明Pep12与PLGA成功结合。