文献：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai, Akihiro Kusumi

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163

Partial cholesterol depletion (using methyl-β-cyclodextrin, MβCD; 60 61, 68) significantly reduced (rather than increased) the DeffMACRO values of both Cy3-DOPE and Bdp-Chol, by 34 and 9.1%, respectively (Table 1). These results are consistent with most of the previous findings, in which partial cholesterol depletion generally did not increase the DeffMACRO values of transmembrane and GPI-anchored proteins as well as cytoplasmic lipid-anchored molecules 48, 69-71, and the diffusion of raft molecules was not slower than that of non-raft molecules 69. Previously (until the report by Kenworthy et al. 69), these results were considered to be at variance with the general concept that raft-associated molecules diffuse more slowly, as compared to non-raft-associated molecules.

部分胆固醇耗竭（使用甲基-β-环糊精，MβCD；60 61，68）显著降低（而不是增加）了Cy3-DOPE和Bdp-Chol的DeffMACRO值，分别降低了34%和9.1%。

这些结果与之前的大多数发现一致，其中部分胆固醇耗竭通常不会增加跨膜和GPI锚定蛋白以及细胞质脂质锚定分子48、69-71的DeffMACRO值，筏分子的扩散并不比非筏分子69慢。此前（直到Kenworthy等人69的报告），这些结果被认为与筏相关分子比非筏相关分子扩散更慢的一般概念不一致。

因此，我们可以得出结论，富含胆固醇的筏结构域不会降低PtK2细胞PM中筏相关（如Bdp-Chol）或非筏相关（如Cy3-DOPE）分子的宏观扩散速率。

相关推荐产品：

DLPE-PEG-SC

DLPE-ICG

Palmitic acid-PEG-SC

DSPE-PEG-TRITC

RB-PEG-DSPE，DSPE-PEG-RB

ICG-PEG-DLPE

ICG-PEG-DMPE

ICG-PEG-DOPE

ICG-PEG-DPPE

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