文献：具有增强粘液渗透性的牛奶外泌体可用于口服递送siRNA

链接：https://pubs.rsc.org/en/content/articlehtml/2020/bm/d0bm01497d

作者：Matthew R. Warren a、 Chenzhen 张 a、 Armin Vedadghavami a、 Krister Bokvist b、 Pradeep K. Dhal c和 Ambika G. Bajpayee * ad

节选：

荧光共轭 DSPE-PEG-叠氮化物的制备。 使用DSPE-PEG-叠氮化物（以下简称DPA）在 mExo 表面形成疏水插入 PEG 涂层。首先将 DPA 以 2 mg mL −1的浓度溶解在 DMSO 中，然后逐滴添加到 1× PBS 中并不断搅拌，使 DPA 的最终浓度为 0.02 mg mL −1（7 μM），从而制备水性储备溶液。对于涉及 DPA 浓度量化的研究（即测量 mExo 表面负载和锚稳定性研究），DPA通过叠氮化物-DBCO 点击化学反应与荧光部分 DBCO-Cy5 结合。将 DBCO-Cy5（DMSO 中浓度为 1 mg mL −1）逐滴添加到水性 DPA 储备液中，不断搅拌，使最终摩尔比为 1 [细空格（1/6-em）]：[细空格（1/6-em）]4。然后在室温下轻轻摇晃该溶液以促进点击反应点击反应后，将含有DPA-Cy5和过量DBCO-Cy5的溶液直接添加到外泌体样品中进行插入。

译文：

Preparation of fluorescent-conjugated DSPE-PEG-Azide. DSPE-PEG-Azide (hereby DPA) was utilized to enable a hydrophobically-inserted PEG coating on the mExo surface. An aqueous stock solution was prepared by first dissolving DPA in DMSO at 2 mg mL−1, followed by dropwise addition to 1× PBS with continuous stirring for a final DPA concentration of 0.02 mg mL−1 (7 μM). For studies involving quantification of DPA concentration (i.e. measurement of mExo surface loading and anchor stability studies), DPA was conjugated to the fluorescent moiety DBCO-Cy5 via the azide-DBCO click chemistry reaction. DBCO-Cy5 (1 mg mL−1 in DMSO) was added dropwise with continuous stirring to aqueous DPA stock for a 1[thin space (1/6-em)]:[thin space (1/6-em)]4 final molar ratio. This solution was then incubated for 6 h at room temperature with light shaking to facilitate the click reaction. After the click reaction, the solution containing DPA-Cy5 and excess DBCO-Cy5 was added directly to exosome samples for insertion.

西安齐岳生物提供相关产品：

DSPE-PEG-Cy5

DSPE-PEG-mAb 2C5（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-PEG-OPSS

Cy5-DSPE

DSPE-PEG-Tetrazine

DSPE-PEG-DA

Asp6-PEG-DSPE

DSPE-PEG2000-NGR

Xpeptide-PEG-DSPE

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