文献：Surface Modification of Erythrocytes with Lipid Anchors: Structure–Activity Relationship for Optimal Membrane Incorporation, in vivo Retention, and Immunocompatibility

作者：Hanmant Gaikwad, Guankui Wang, Yue Li, David Bourne, Dmitri Simberg

文献链接：https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163

Thus, RBCs labeled with diacyl glycerol derivative Cy3-C12 and phospholipid Cy3-DSPE showed much faster removal than stable DiI-C12. To compare the stability of different lipids in vitro, we measured the fluorescence of labeled RBCs after incubation in mouse serum for 3 h. According to Figure  5A, DiI-C18, DiI-C18:2, DiI-PEG3400Mtz, DiI-C12, Cy3-C12, and Cy3-cholesterol RBCs showed less than 15% loss in MFI at 3 h. At the same time, Cy3-DSPE RBCs showed over 60% loss of MFI. Confocal microscopy showed that Cy3-DSPE and DiI-C18 had similar uniform labeling of RBCs prior to incubation in serum (Figure 5B). After 3 h incubation in serum, there was five times more fluorescence released in serum from Cy3-DSPE RBCs than from DiI-C18 RBCs (Figure 5C). Thin layer chromatography (TLC) analysis showed the presence of intact Cy3-DSPE along with some degradation products (Figure 5D). While 3 h time incubation is shorter than the in vivo longevity of some of the lipids (Figure 3B), the data indicate the removal of the phospholipid from RBC membrane through interaction with serum.

用二酰基甘油衍生物Cy3-C12和磷脂Cy3-DSPE标记的红细胞显示出比稳定的DiI-C12更快的去除速度。比较不同脂质在 在体外，我们测量了标记的红细胞在小鼠血清中孵育3小时后的荧光 h.DiI-C18、DiI-C18:2、DiI-PEG3400Mtz、DiI-C12、Cy3-C12和Cy3胆固醇红细胞在3℃时MFI损失小于15% h.同时，Cy3-DSPE红细胞MFI损失超过60%。

共聚焦显微镜显示，Cy3-DSPE和DiI-C18在血清中孵育前对红细胞具有相似的均匀标记（图5B）。3之后 在血清中孵育h后，Cy3-DSPE红细胞在血清中释放的荧光是DiI-C18红细胞的五倍。

薄层色谱（TLC）分析显示存在完整的Cy3-DSPE和一些降解产物（图5D）。虽然3 h孵化时间短于in 一些脂质的体内寿命，数据表明磷脂通过与血清的相互作用从红细胞膜上去除。

相关推荐：

Cy3-DPPE

Cy3-DSPE

Cy3-PEG-DOPE

Cy3-PEG-DPPE

Cy5-DOPE

Cy5-DPPE

Cy5-DSPE

Cy5-PEG-DOPE

Cy5-PEG-DPPE

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