N3-PEG-COOH接枝壳聚糖纳米颗粒的制备与表征-UDP糖丨MOF丨金属有机框架丨聚集诱导发光丨荧光标记推荐西安齐岳生物

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N3-PEG-COOH接枝壳聚糖纳米颗粒的制备与表征

发布时间：2025-07-16 作者：zyl 分享到：

标题功能型聚乙二醇化壳聚糖纳米颗粒的制备和表征

作者肖巨颖; 赵霞; 林园; 苏朝晖

文献链接：https://openurl.ebsco.com/EPDB%3Agcd%3A14%3A17421374/detailv2?sid=ebsco%3Aplink%3Acrawler&id=ebsco%3Adoi%3A10.19894%2Fj.issn.1000-0518.240425&link_origin=none

摘要：

In the present study, Carboxyl and azide terminated polyethylene (N3-PEG-COOH) with different relative molecular mass of 500 and 1000 were grafted onto the chitosan (CS) backbones through amide reaction, followed by the preparation of chitosan-polyethylene glycol (CS-PEG) nanoparticles with controllable size and zeta potential via sodium tripolyphosphate (TPP) ionotropic gelation. Characterized by Fourier transform infrared spectrometer (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and thermal gravimetric analyzer (TGA) confirmed the successful incorporation of PEG, and the structures of the nanoparticles were analyzed by transmission electron microscopy (TEM) and nanoparticle size and zeta potential analyzer. The results demonstrated that when m (CS) ∶m (TPP) is 3.33∶1, CS nanoparticles show excellent structural stability. Based on this, increasing the PEG grafting density further reduces the particle size and surface potential of CS-PEG nanoparticles. Moreover, the chemical reactivity of azide in CS-PEG nanoparticles was proved by the click reaction with alkyne-modified fluorescent molecule (5-FAM-alkyne), showing their potential as platform for target drug delivery, fluorescent probes and other biomedical applications.

N3-PEG-COOH

在本研究中，通过酰胺反应将相对分子质量为500和1000的羧基和叠氮封端的聚乙烯（N3-PEG-COOH）接枝到壳聚糖（CS）骨架上，然后通过三聚磷酸钠（TPP）离子凝胶化制备尺寸和ζ电位可控的壳聚糖-聚乙二醇（CS-PEG）纳米粒子。通过傅里叶变换红外光谱仪（FT-IR）、核磁共振光谱（NMR）和热重分析仪（TGA）的表征证实了PEG的成功掺入，并通过透射电子显微镜（TEM）和纳米粒子尺寸和ζ电位分析仪分析了纳米粒子的结构。

结果表明，当m（CS）∶m（TPP）为3.33∶1时，CS纳米粒子表现出优异的结构稳定性。基于此，增加PEG接枝密度进一步降低了CS-PEG纳米颗粒的粒径和表面电位。此外，通过与炔烃修饰的荧光分子（5-FAM-炔烃）的点击反应，证明了叠氮化物在CS-PEG纳米颗粒中的化学反应性，显示了它们作为靶向药物递送、荧光探针和其他生物医学应用平台的潜力。