文献:Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy
作者:Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie
文献链接:https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801
摘要:
1H NMR was determined to confirm the synthesis of FA-PEG-PEI (the spectrum and relevant chemical structural formula were shown in Figure 1). The characteristic peaks from 2.0 to 3.0 ppm (k, j) were attributed to -CH2CH2NH- group of PEI, the peaks at 3.66 ppm (h) were referred to -CH2CH2O- group of PEG, and the peaks from 6.5 to 9.0 ppm (a, b, c) were related to the H protons of benzene ring in FA [25]. The data suggested a successful combination of FA-PEG-PEI.
The possible functional groups responsible for the synthesis of FA-PEG-PEI were further confirmed by FT-IR spectroscopy. Meanwhile, the FT-IR spectra of FA, NH2-PEG-OH, and NH2-PEG-COOH were also examined. The terminal hydroxyl groups of NH2-PEG-OH were of carboxylation and then combined with the amino groups of PEI via amidation reaction. As shown in Figure 2, the FT-IR spectra of terminal hydroxyl groups of (b) NH2-PEG-OH showed the presence of stretching vibration peak of -OH at 3440 cm−1, which shifted to a new stretching vibration peak of -C=O at 1629 cm−1 in (c) NH2-PEG-COOH after carboxylation. The FT-IR spectra of (d) FA-PEG-PEI displayed a broad peak at 3350–3250 cm−1, which refers to -NH2 and -NH- groups of PEI. The peaks at 1606–1460 cm−1 correspond to the benzene ring of FA. In addition, the peak at 1110 cm−1 is attributed to the symmetrical stretching vibration of -C-O-C- group in PEG, which declares the conjugation of FA-PEG-PEI.
测定1H NMR以确认FA-PEG-PEI的合成。特征峰值在2.0到3.0之间 ppm(k,j)归因于PEI的-CH2CH2NH-基团,峰值在3.66 ppm(h)是指PEG的-CH2CH2O-基团,峰在6.5至9.0之间 ppm(a,b,c)与FA中苯环的H质子有关。数据表明,FA-PEG-PEI的组合是成功的。
FT-IR光谱进一步证实了可能负责FA-PEG-PEI合成的官能团。同时,还研究了FA、NH2-PEG-OH和NH2-PEG-COOH的傅里叶变换红外光谱。
NH2-PEG-OH的末端羟基被羧化,然后通过酰胺化反应与PEI的氨基结合。如图2所示,(b)NH2-PEG-OH末端羟基的FT-IR光谱显示,在3440处存在-OH的伸缩振动峰 cm-1,在1629处移动到新的拉伸振动峰-C=O 羧化后,在(c)NH2-PEG-COOH中加入cm-1。(d)FA-PEG-PEI的FT-IR光谱在3350-3250处显示了一个宽峰 cm-1表示PEI的-NH2和-NH-基团。1606年至1460年的峰值 cm-1对应于FA的苯环。此外,1110处的峰 cm-1归因于PEG中-C-O-C-基团的对称伸缩振动,这宣告了FA-PEG-PEI的共轭。
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