文献：PLL-PEG-PLL的制备及其在DNA封装中的应用

链接：https://link.springer.com/article/10.1007/s11426-006-2006-9

作者：杨子刚,周芬,袁建军,马立新,翟超&程士元

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通过对PLL(Z)-PEG-PLL(Z)中氨基进行脱保护，合成了PLL-PEG-PLL。研究了PLL-PEG-PLL与DNA形成的聚离子复合物（PIC）胶束的性质。结果表明，PIC的核心是由PLL与DNA通过静电相互作用形成的，PEG为PIC的外壳，因此PIC胶束在水相中均匀分布，即使在中性条件下也不会沉淀，具有良好的稳定性，这在基因*中具有重要意义。同时，结果表明PLL-PEG-PLL可以对DNA进行缩合，且PLL嵌段越长，缩合效果越好，并且DNA的*核酸酶活性也显著提高。PIC呈球形，直径为10~35 nm，易于被细胞摄取。体外转染实验表明，所包裹的DNA可以在SF9细胞系中表达。

PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.

西安齐岳生物提供相关产品：

PLL-g-PEG-Biotin

PLL-g-PEG-N3

PLL-PEG

PLL-PEG-N3

PLL-PEG-OPSS

Poly(L-Aspartic acid)-PEGs

Poly(L-Ornithine)-MPEG

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